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Part:BBa_K1875004:Design

Designed by: Jeffrey Marano   Group: iGEM16_BostonU   (2016-10-11)


A 20 bp target sequence and PAM to make guide operator 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This guide was designed based on its orthogonality to the human genome (hg19)


Source

This guide was synthesized by IDT

References